Research Letters

Characterisation of the arsenic resistance genes in Bacillus sp. UWC isolated from maturing fly ash acid mine drainage neutralised solids

Wicleffe Musingarimi, Marla Tuffin, Donald Cowan
South African Journal of Science | Vol 106, No 1/2 | a17 | DOI: https://doi.org/10.4102/sajs.v106i1/2.17 | © 2010 Wicleffe Musingarimi, Marla Tuffin, Donald Cowan | This work is licensed under CC Attribution 4.0
Submitted: 18 January 2010 | Published: 17 March 2010

About the author(s)

Wicleffe Musingarimi, University of the Western Cape, South Africa
Marla Tuffin, University of the Western Cape, South Africa
Donald Cowan, University of the Western Cape, South Africa

Abstract

An arsenic resistant Bacillus sp. UWC was isolated from fly ash acid mine drainage (FA-AMD) neutralised solids. A genomic library was prepared and screened in an arsenic sensitive mutant Escherichia coli strain for the presence of arsenic resistance (ars) genes. Sequence analysis of a clone conferring resistance to both sodium arsenite and sodium arsenate revealed homologues to the arsR (regulatory repressor), arsB (membrane located arsenite pump), arsC (arsenate reductase), arsD (second regulatory repressor and a metallochaperone) and arsA (ATPase) genes from known arsenic resistance operons. The Bacillus sp. UWC arsRBCDA genes were shown to be arranged in an unusual manner with the arsDA genes immediately downstream of arsC.

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Crossref Citations

1. Physical, Chemical, and Biological Methods for the Removal of Arsenic Compounds
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doi: 10.1155/2014/503784