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Next generation shotgun sequencing and the challenges of de novo genome assembly

Stephen Schlebusch, Nicola Illing
South African Journal of Science | Vol 108, No 11/12 | a1256 | DOI: https://doi.org/10.4102/sajs.v108i11/12.1256 | © 2012 Stephen Schlebusch, Nicola Illing | This work is licensed under CC Attribution 4.0
Submitted: 03 May 2012 | Published: 31 October 2012

About the author(s)

Stephen Schlebusch, Department of Molecular and Cell Biology, University of Cape Town, Cape Town, South Africa
Nicola Illing, Department of Molecular and Cell Biology, University of Cape Town, Cape Town, South Africa


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Abstract

Sequencing the genomes of the many scientifically fascinating plants and animals found in South Africa is fast becoming a viable option as a result of the rapid and sustained drop in the cost of next generation sequencing over the last five years. However, the processing and assembly of the sequence data produced is not trivial. There are several factors which need to be taken into consideration when planning a strategy to assemble genome sequence data de novo. This paper reviews the advances and the challenges in two of the most rapidly developing areas of the field: the sequencing technology and the software programs used to assemble de novo the sequence data generated by these technologies into a genome.


Keywords

de novo genome assembly; next generation sequencing; de Bruijn; whole genome sequencing

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