Research Articles

Femtosecond pump probe spectroscopy for the study of energy transfer of light-harvesting complexes from extractions of spinach leaves

S. Ombinda-Lemboumba, A. du du Plessis, R.W. Sparrow, P. Molukanele, L.R. Botha, E.G. Rohwer, C.M. Steenkamp, L. van Rensburg
South African Journal of Science | Vol 105, No 9/10 | a115 | DOI: https://doi.org/10.4102/sajs.v105i9/10.115 | © 2010 S. Ombinda-Lemboumba, A. du du Plessis, R.W. Sparrow, P. Molukanele, L.R. Botha, E.G. Rohwer, C.M. Steenkamp, L. van Rensburg | This work is licensed under CC Attribution 4.0
Submitted: 20 January 2010 | Published: 20 January 2010

About the author(s)

S. Ombinda-Lemboumba, CSIR National Laser Centre, Laser Research Institute, Department of Physics, University of Stellenbosch, South Africa
A. du du Plessis, CSIR National Laser Centre, Laser Research Institute, Department of Physics, University of Stellenbosch, South Africa
R.W. Sparrow, CSIR National Laser Centre, Laser Research Institute, Department of Physics, University of Stellenbosch, South Africa
P. Molukanele, CSIR National Laser Centre, cCSIR Biosciences, South Africa
L.R. Botha, CSIR National Laser Centre, Laser Research Institute, Department of Physics, University of Stellenbosch, South Africa
E.G. Rohwer, Laser Research Institute, Department of Physics, University of Stellenbosch, South Africa
C.M. Steenkamp, Laser Research Institute, Department of Physics, University of Stellenbosch, South Africa
L. van Rensburg, dEnvironmental Science and Management, North-West University, South Africa

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Abstract

Measurements of ultrafast transient processes, of temporal durations in the picosecond and femtosecond regime, are made possible by femtosecond pump probe transient absorption spectroscopy. Such an ultrafast pump probe transient absorption setup has been implemented at the CSIR National Laser Centre and has been applied to investigate energy transfer processes in different parts of photosynthetic systems. In this paper we report on our first results obtained with Malachite green as a benchmark. Malachite green was chosen because the lifetime of its excited state is well known. We also present experimental results of the ultrafast energy transfer of light-harvesting complexes in samples prepared from spinach leaves. Various pump wavelengths in the range 600–680 nm were used; the probe was a white light continuum spanning 420–700 nm. The experimental setup is described in detail in this paper. Results obtained with these samples are consistent with those expected and achieved by other researchers in this field.

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